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KMID : 0895820180280020037
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Journal of Oriental Rehabilitation Medicine
2018 Volume.28 No. 2 p.37 ~ p.45
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Anti-inflammatory Effects of Gagamtongsoon-San Extract on Lipopolysaccharide(LPS)-Induced Inflammation in RAW264.7 Cells
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Lee Soo-Hwan
Kim Soon-Joong
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Abstract
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Objectives: This study was designed to investigate whether the Gagamtongsoon-San (GT) has an inhibitory effect and its mechanisms are associated with the iNOS and COX-2.
Methods: Cytotoxic activity of GT extract on RAW264.7 cells was evaluated by using 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) solution. Inflammatory condition was induced by LPS. NO production was measured using Griess reagent system. The expressions of iNOS and COX-2 mRNA and protein were determined by realtime PCR. The concentrations of PGE2 were measured by an enzyme immunoassay (EIA).
Results: The GT does not impair the cell viability in tested concentration 500 μg/ml or below. GT significantly reduced the NO production in a dose-dependent manner. GT 500 μg/ml also suppressed LPS-induced mRNA expressions of iNOS and COX-2. GT 500 μg/ml reduced the PGE2 secretion in LPS induced RAW264.7 cells.
Conclusions: These outcomes show that GT extract has an anti-inflammatory activities. And also this conclusion can be the data that supports the GT’s anti-inflammatory effect objectively.
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KEYWORD
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Gagamtongsoon-San(GT), Anti-inflammatory, RAW264.7, LPS
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